Journal: ACS Omega
Article Title: Bioinformatics Analysis and Experimental Verification Define Different Angiogenesis Subtypes in Endometrial Carcinoma and Identify a Prognostic Signature
doi: 10.1021/acsomega.4c03034
Figure Lengend Snippet: Expression levels of selected angiogenesis-related hub genes. (A) Differential transcription levels of IHH, TUBG1, EEF2, and MECP2 in UCEC cell lines and hEECs. (B) Violin diagrams of differential transcript levels of IHH, TUBG1, EEF2, and MECP2 in UCEC tissues and paratumor tissues. (C) Comparison of the protein expression levels of IHH, TUBG1, EEF2, and MECP2 between UCEC and paratumor by immunohistochemistry staining and analyzed by calculating average optical density (AOD: IOD/area). (D) Representative immunohistochemistry staining images. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001, and ns no significance.
Article Snippet: Human endometrial cancer cell lines (RL-952, KLE, HEC-1B, HEC-1A, and Ishikawa), normal human endometrial epithelial cells (hEECs), and human umbilical vein endothelial cells (HUVECs) were purchased from the American Type Culture Collection (ATCC).
Techniques: Expressing, Comparison, Immunohistochemistry, Staining